Serum Protein Concentration in the African Free-Ranging Chicken of Different Ages and Sexes.
Keywords:
spectrophotometer, spectrophotometry, Albumin and globulinAbstract
Serum total protein, albumin and globulin concentrations were determined in 63 free-ranging chickens aged 1.5 to 10 months old and of different sexes using refractometric (non-temperature-compensated) and spectrophotometric methods. Serum albumin concentrations were determined spectrophotometrically and globulin calculated. The purpose was to determine the trend of serum proteins with age and sex, and at the same time compare the refractometric and spectrophotometric serum total protein values. Significant differences in serum total protein (P<0.001), albumin (P<0.01) and globulin (P<0.05) concentrations were observed between age groups but not between male and female birds. Spectrophotometric values were significantly (P<0.001) greater than refractometric values but highly correlated (r=0.85)
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